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murine e2f8  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology murine e2f8
    Figure 2 E2f7 and <t>E2f8</t> promote TGC endocycles. (a) NanoString analysis of TGC-specific E2f4–6 (left panel) and E2f7/E2f8 (right panel) expression in laser-capture-microdissected wild-type cells; n = 2 placentae analysed per time point. (b) Immunohistochemistry demonstrating E2F7 (top left) and E2F8 (bottom left) expression in wild-type E10.5 TGCs but not mutant controls (right panels). The arrows point to selected TGCs. (c) Representative H&E-stained sections of E10.5 control and E2f7−/−;E2f8−/−(78dko) placentae. Inset, higher magnification of the outlined area, showing a 78dko TGC in metaphase. (d) Feulgen quantification of genome ploidy in E10.5 TGCs; n = 3 placentae analysed per genetic group. (e–g) Immunostaining and quantification of S and M phase proteins in E10.5 control and 78dko TGCs. The arrows point to TGCs. n = 3 placentae analysed per genetic group. (h) Co-immunofluorescence microscopy showing E10.5 78dko TGCs in anaphase (left, P-H3) and metaphase (right, PL-1). DAPI stained total DNA. (i) Representative confocal images of nuclei in E10.5 control (top right) and 78dko TGCs
    Murine E2f8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+e2f8/E2F-8+Antibody/pm23064266-501-39-58
    Average 92 stars, based on 12 article reviews
    murine e2f8 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Canonical and atypical E2Fs regulate the mammalian endocycle."

    Article Title: Canonical and atypical E2Fs regulate the mammalian endocycle.

    Journal: Nature cell biology

    doi: 10.1038/ncb2595

    Figure 2 E2f7 and E2f8 promote TGC endocycles. (a) NanoString analysis of TGC-specific E2f4–6 (left panel) and E2f7/E2f8 (right panel) expression in laser-capture-microdissected wild-type cells; n = 2 placentae analysed per time point. (b) Immunohistochemistry demonstrating E2F7 (top left) and E2F8 (bottom left) expression in wild-type E10.5 TGCs but not mutant controls (right panels). The arrows point to selected TGCs. (c) Representative H&E-stained sections of E10.5 control and E2f7−/−;E2f8−/−(78dko) placentae. Inset, higher magnification of the outlined area, showing a 78dko TGC in metaphase. (d) Feulgen quantification of genome ploidy in E10.5 TGCs; n = 3 placentae analysed per genetic group. (e–g) Immunostaining and quantification of S and M phase proteins in E10.5 control and 78dko TGCs. The arrows point to TGCs. n = 3 placentae analysed per genetic group. (h) Co-immunofluorescence microscopy showing E10.5 78dko TGCs in anaphase (left, P-H3) and metaphase (right, PL-1). DAPI stained total DNA. (i) Representative confocal images of nuclei in E10.5 control (top right) and 78dko TGCs
    Figure Legend Snippet: Figure 2 E2f7 and E2f8 promote TGC endocycles. (a) NanoString analysis of TGC-specific E2f4–6 (left panel) and E2f7/E2f8 (right panel) expression in laser-capture-microdissected wild-type cells; n = 2 placentae analysed per time point. (b) Immunohistochemistry demonstrating E2F7 (top left) and E2F8 (bottom left) expression in wild-type E10.5 TGCs but not mutant controls (right panels). The arrows point to selected TGCs. (c) Representative H&E-stained sections of E10.5 control and E2f7−/−;E2f8−/−(78dko) placentae. Inset, higher magnification of the outlined area, showing a 78dko TGC in metaphase. (d) Feulgen quantification of genome ploidy in E10.5 TGCs; n = 3 placentae analysed per genetic group. (e–g) Immunostaining and quantification of S and M phase proteins in E10.5 control and 78dko TGCs. The arrows point to TGCs. n = 3 placentae analysed per genetic group. (h) Co-immunofluorescence microscopy showing E10.5 78dko TGCs in anaphase (left, P-H3) and metaphase (right, PL-1). DAPI stained total DNA. (i) Representative confocal images of nuclei in E10.5 control (top right) and 78dko TGCs

    Techniques Used: Expressing, Immunohistochemistry, Mutagenesis, Staining, Control, Immunostaining, Microscopy

    Figure 4 Canonical activator and atypical repressor E2Fs regulate key transcriptional networks coordinating endocycles. (a) Heat map of approximately 4,500 differentially expressed genes in weaning age (3 wk) livers. Class I: genes regulated by E2F7 and E2F8. Class II: genes regulated by either E2F7 or E2F8. Class III: genes synergistically regulated by E2F7/E2F8. n = 3–4 livers analysed per genetic group. Control, E2f7f / f;E2f8f / f; Alb-78dko, Alb–cre;E2f7f / f;E2f8f/ f; Alb-8ko, Alb–cre;E2f8f / f; Alb-7ko, Alb–cre;E2f7f / f. (b) Top, heat map of TGC gene expression in a custom NanoString mRNA code set. RNA was isolated from laser-capture-microdissected E10.5 TGCs in frozen
    Figure Legend Snippet: Figure 4 Canonical activator and atypical repressor E2Fs regulate key transcriptional networks coordinating endocycles. (a) Heat map of approximately 4,500 differentially expressed genes in weaning age (3 wk) livers. Class I: genes regulated by E2F7 and E2F8. Class II: genes regulated by either E2F7 or E2F8. Class III: genes synergistically regulated by E2F7/E2F8. n = 3–4 livers analysed per genetic group. Control, E2f7f / f;E2f8f / f; Alb-78dko, Alb–cre;E2f7f / f;E2f8f/ f; Alb-8ko, Alb–cre;E2f8f / f; Alb-7ko, Alb–cre;E2f7f / f. (b) Top, heat map of TGC gene expression in a custom NanoString mRNA code set. RNA was isolated from laser-capture-microdissected E10.5 TGCs in frozen

    Techniques Used: Control, Gene Expression, Isolation

    Figure 5 Atypical repressors E2F7/E2F8 directly bind gene targets involved in endocycle control. (a) Immunoblot of transfected HepG2 cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (b) Representative ChIP assay in transfected HepG2 cells with anti-Flag antibodies demonstrating enhanced occupancy of Flag-tagged E2F7 and E2F8 proteins on E2F-binding sites in the promoter of G1/S (E2f1 and Ccne2) and G2/M (Ccna2 and Chek1) genes. The tubulin (Tub) gene, a non-E2F target, demonstrates specific recruitment of Flag-tagged E2F7 and E2F8 to target promoters containing consensus E2F-binding sequences. n = 3 independent transfection–ChIP experiments performed. (c) Immunoblot of transfected Rcho-1 trophoblast stem (TS) cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (d) Representative ChIP assays in transfected Rcho-1 trophoblast stem cells of G1/S and
    Figure Legend Snippet: Figure 5 Atypical repressors E2F7/E2F8 directly bind gene targets involved in endocycle control. (a) Immunoblot of transfected HepG2 cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (b) Representative ChIP assay in transfected HepG2 cells with anti-Flag antibodies demonstrating enhanced occupancy of Flag-tagged E2F7 and E2F8 proteins on E2F-binding sites in the promoter of G1/S (E2f1 and Ccne2) and G2/M (Ccna2 and Chek1) genes. The tubulin (Tub) gene, a non-E2F target, demonstrates specific recruitment of Flag-tagged E2F7 and E2F8 to target promoters containing consensus E2F-binding sequences. n = 3 independent transfection–ChIP experiments performed. (c) Immunoblot of transfected Rcho-1 trophoblast stem (TS) cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (d) Representative ChIP assays in transfected Rcho-1 trophoblast stem cells of G1/S and

    Techniques Used: Control, Western Blot, Transfection, Expressing, Binding Assay

    Figure 7 Cyclin A ablation reinstates genome ploidy of E2f7/E2f8-deficient TGCs and hepatocytes. (a) Images of H&E-stained E10.5 TGCs. The combined loss of Ccna1 and Ccna2 (Plf-A12dko) led to TGCs similar in appearance to control TGCs. The further loss of Ccna1 and Ccna2 in E2f7/E2f8-deficient TGCs (Plf-A1278qko) led to TGCs with a normal (wild-type-like) appearance, in contrast to Plf-78dko TGCs that seemed small or were binucleated (yellow arrows). The lower left insets show higher magnifications of the areas outlined in the main panels. The lower right insets show higher magnifications of the areas outlined in the main panels. (b) Feulgen quantification of genome ploidy in E10.5 TGCs. The intensities (that is, estimated genome content) of 120–140 TGCs were quantified per placenta sample, with n placenta samples analysed per genetic group as indicated. A number of TGCs quadruply deficient for Ccna1, Ccna2, E2f7 and E2f8 reach ploidy levels of 128C and 256C. Control, E2f7f / f;E2f8f / f; Plf-A12, Plfcre/+;Ccna1−/−;Ccna2f / f; Plf-78dko, Plfcre/+;E2f7f / f;E2f8f / f;Plf-A1278qko, Plfcre/+;Ccna1−/−;Ccna2f / f; E2f7f / f;E2f8f / f. (c) Images of H&E-stained 3-month-old liver sections,
    Figure Legend Snippet: Figure 7 Cyclin A ablation reinstates genome ploidy of E2f7/E2f8-deficient TGCs and hepatocytes. (a) Images of H&E-stained E10.5 TGCs. The combined loss of Ccna1 and Ccna2 (Plf-A12dko) led to TGCs similar in appearance to control TGCs. The further loss of Ccna1 and Ccna2 in E2f7/E2f8-deficient TGCs (Plf-A1278qko) led to TGCs with a normal (wild-type-like) appearance, in contrast to Plf-78dko TGCs that seemed small or were binucleated (yellow arrows). The lower left insets show higher magnifications of the areas outlined in the main panels. The lower right insets show higher magnifications of the areas outlined in the main panels. (b) Feulgen quantification of genome ploidy in E10.5 TGCs. The intensities (that is, estimated genome content) of 120–140 TGCs were quantified per placenta sample, with n placenta samples analysed per genetic group as indicated. A number of TGCs quadruply deficient for Ccna1, Ccna2, E2f7 and E2f8 reach ploidy levels of 128C and 256C. Control, E2f7f / f;E2f8f / f; Plf-A12, Plfcre/+;Ccna1−/−;Ccna2f / f; Plf-78dko, Plfcre/+;E2f7f / f;E2f8f / f;Plf-A1278qko, Plfcre/+;Ccna1−/−;Ccna2f / f; E2f7f / f;E2f8f / f. (c) Images of H&E-stained 3-month-old liver sections,

    Techniques Used: Staining, Control

    Related Articles

    Incubation:

    Article Title: Canonical and atypical E2Fs regulate the mammalian endocycle.
    Article Snippet: .. After antigen retrieval using Target Retrieval Solution (DAKO S1699), 5 μm deparaffinized sections of placenta or liver tissues were incubated with primary antibodies at 4 ◦C overnight against E2F7 1:100 (Abcam ab56022), E2F8 1:50 (polyclonal against residues 576–595 of murine E2F8), BrdU 1:100 (DAKOMO-0744), Ki67 1:100 (BD Pharmingen 550609), phospho-histone 3 (Ser10) 1:100 (Millipore 06-570), cyclin A2 1:100 (Santa Cruz sc-596), cyclin B1 1:100 (Santa Cruz sc-752), E-cadherin 1:100 (Abcam ab53033), p57Kip2 1:100 (Santa Cruz sc-8298; refs 56,57) and placental lactogen 1:200 (PL-1, F. Talamantes). ..



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    murine e2f8  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology murine e2f8
    Figure 2 E2f7 and <t>E2f8</t> promote TGC endocycles. (a) NanoString analysis of TGC-specific E2f4–6 (left panel) and E2f7/E2f8 (right panel) expression in laser-capture-microdissected wild-type cells; n = 2 placentae analysed per time point. (b) Immunohistochemistry demonstrating E2F7 (top left) and E2F8 (bottom left) expression in wild-type E10.5 TGCs but not mutant controls (right panels). The arrows point to selected TGCs. (c) Representative H&E-stained sections of E10.5 control and E2f7−/−;E2f8−/−(78dko) placentae. Inset, higher magnification of the outlined area, showing a 78dko TGC in metaphase. (d) Feulgen quantification of genome ploidy in E10.5 TGCs; n = 3 placentae analysed per genetic group. (e–g) Immunostaining and quantification of S and M phase proteins in E10.5 control and 78dko TGCs. The arrows point to TGCs. n = 3 placentae analysed per genetic group. (h) Co-immunofluorescence microscopy showing E10.5 78dko TGCs in anaphase (left, P-H3) and metaphase (right, PL-1). DAPI stained total DNA. (i) Representative confocal images of nuclei in E10.5 control (top right) and 78dko TGCs
    Murine E2f8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+e2f8/E2F-8+Antibody/pm23064266-501-39-58
    Average 92 stars, based on 1 article reviews
    murine e2f8 - by Bioz Stars, 2026-09
    92/100 stars
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    Figure 2 E2f7 and E2f8 promote TGC endocycles. (a) NanoString analysis of TGC-specific E2f4–6 (left panel) and E2f7/E2f8 (right panel) expression in laser-capture-microdissected wild-type cells; n = 2 placentae analysed per time point. (b) Immunohistochemistry demonstrating E2F7 (top left) and E2F8 (bottom left) expression in wild-type E10.5 TGCs but not mutant controls (right panels). The arrows point to selected TGCs. (c) Representative H&E-stained sections of E10.5 control and E2f7−/−;E2f8−/−(78dko) placentae. Inset, higher magnification of the outlined area, showing a 78dko TGC in metaphase. (d) Feulgen quantification of genome ploidy in E10.5 TGCs; n = 3 placentae analysed per genetic group. (e–g) Immunostaining and quantification of S and M phase proteins in E10.5 control and 78dko TGCs. The arrows point to TGCs. n = 3 placentae analysed per genetic group. (h) Co-immunofluorescence microscopy showing E10.5 78dko TGCs in anaphase (left, P-H3) and metaphase (right, PL-1). DAPI stained total DNA. (i) Representative confocal images of nuclei in E10.5 control (top right) and 78dko TGCs

    Journal: Nature cell biology

    Article Title: Canonical and atypical E2Fs regulate the mammalian endocycle.

    doi: 10.1038/ncb2595

    Figure Lengend Snippet: Figure 2 E2f7 and E2f8 promote TGC endocycles. (a) NanoString analysis of TGC-specific E2f4–6 (left panel) and E2f7/E2f8 (right panel) expression in laser-capture-microdissected wild-type cells; n = 2 placentae analysed per time point. (b) Immunohistochemistry demonstrating E2F7 (top left) and E2F8 (bottom left) expression in wild-type E10.5 TGCs but not mutant controls (right panels). The arrows point to selected TGCs. (c) Representative H&E-stained sections of E10.5 control and E2f7−/−;E2f8−/−(78dko) placentae. Inset, higher magnification of the outlined area, showing a 78dko TGC in metaphase. (d) Feulgen quantification of genome ploidy in E10.5 TGCs; n = 3 placentae analysed per genetic group. (e–g) Immunostaining and quantification of S and M phase proteins in E10.5 control and 78dko TGCs. The arrows point to TGCs. n = 3 placentae analysed per genetic group. (h) Co-immunofluorescence microscopy showing E10.5 78dko TGCs in anaphase (left, P-H3) and metaphase (right, PL-1). DAPI stained total DNA. (i) Representative confocal images of nuclei in E10.5 control (top right) and 78dko TGCs

    Article Snippet: After antigen retrieval using Target Retrieval Solution (DAKO S1699), 5 μm deparaffinized sections of placenta or liver tissues were incubated with primary antibodies at 4 ◦C overnight against E2F7 1:100 (Abcam ab56022), E2F8 1:50 (polyclonal against residues 576–595 of murine E2F8), BrdU 1:100 (DAKOMO-0744), Ki67 1:100 (BD Pharmingen 550609), phospho-histone 3 (Ser10) 1:100 (Millipore 06-570), cyclin A2 1:100 (Santa Cruz sc-596), cyclin B1 1:100 (Santa Cruz sc-752), E-cadherin 1:100 (Abcam ab53033), p57Kip2 1:100 (Santa Cruz sc-8298; refs 56,57) and placental lactogen 1:200 (PL-1, F. Talamantes).

    Techniques: Expressing, Immunohistochemistry, Mutagenesis, Staining, Control, Immunostaining, Microscopy

    Figure 4 Canonical activator and atypical repressor E2Fs regulate key transcriptional networks coordinating endocycles. (a) Heat map of approximately 4,500 differentially expressed genes in weaning age (3 wk) livers. Class I: genes regulated by E2F7 and E2F8. Class II: genes regulated by either E2F7 or E2F8. Class III: genes synergistically regulated by E2F7/E2F8. n = 3–4 livers analysed per genetic group. Control, E2f7f / f;E2f8f / f; Alb-78dko, Alb–cre;E2f7f / f;E2f8f/ f; Alb-8ko, Alb–cre;E2f8f / f; Alb-7ko, Alb–cre;E2f7f / f. (b) Top, heat map of TGC gene expression in a custom NanoString mRNA code set. RNA was isolated from laser-capture-microdissected E10.5 TGCs in frozen

    Journal: Nature cell biology

    Article Title: Canonical and atypical E2Fs regulate the mammalian endocycle.

    doi: 10.1038/ncb2595

    Figure Lengend Snippet: Figure 4 Canonical activator and atypical repressor E2Fs regulate key transcriptional networks coordinating endocycles. (a) Heat map of approximately 4,500 differentially expressed genes in weaning age (3 wk) livers. Class I: genes regulated by E2F7 and E2F8. Class II: genes regulated by either E2F7 or E2F8. Class III: genes synergistically regulated by E2F7/E2F8. n = 3–4 livers analysed per genetic group. Control, E2f7f / f;E2f8f / f; Alb-78dko, Alb–cre;E2f7f / f;E2f8f/ f; Alb-8ko, Alb–cre;E2f8f / f; Alb-7ko, Alb–cre;E2f7f / f. (b) Top, heat map of TGC gene expression in a custom NanoString mRNA code set. RNA was isolated from laser-capture-microdissected E10.5 TGCs in frozen

    Article Snippet: After antigen retrieval using Target Retrieval Solution (DAKO S1699), 5 μm deparaffinized sections of placenta or liver tissues were incubated with primary antibodies at 4 ◦C overnight against E2F7 1:100 (Abcam ab56022), E2F8 1:50 (polyclonal against residues 576–595 of murine E2F8), BrdU 1:100 (DAKOMO-0744), Ki67 1:100 (BD Pharmingen 550609), phospho-histone 3 (Ser10) 1:100 (Millipore 06-570), cyclin A2 1:100 (Santa Cruz sc-596), cyclin B1 1:100 (Santa Cruz sc-752), E-cadherin 1:100 (Abcam ab53033), p57Kip2 1:100 (Santa Cruz sc-8298; refs 56,57) and placental lactogen 1:200 (PL-1, F. Talamantes).

    Techniques: Control, Gene Expression, Isolation

    Figure 5 Atypical repressors E2F7/E2F8 directly bind gene targets involved in endocycle control. (a) Immunoblot of transfected HepG2 cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (b) Representative ChIP assay in transfected HepG2 cells with anti-Flag antibodies demonstrating enhanced occupancy of Flag-tagged E2F7 and E2F8 proteins on E2F-binding sites in the promoter of G1/S (E2f1 and Ccne2) and G2/M (Ccna2 and Chek1) genes. The tubulin (Tub) gene, a non-E2F target, demonstrates specific recruitment of Flag-tagged E2F7 and E2F8 to target promoters containing consensus E2F-binding sequences. n = 3 independent transfection–ChIP experiments performed. (c) Immunoblot of transfected Rcho-1 trophoblast stem (TS) cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (d) Representative ChIP assays in transfected Rcho-1 trophoblast stem cells of G1/S and

    Journal: Nature cell biology

    Article Title: Canonical and atypical E2Fs regulate the mammalian endocycle.

    doi: 10.1038/ncb2595

    Figure Lengend Snippet: Figure 5 Atypical repressors E2F7/E2F8 directly bind gene targets involved in endocycle control. (a) Immunoblot of transfected HepG2 cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (b) Representative ChIP assay in transfected HepG2 cells with anti-Flag antibodies demonstrating enhanced occupancy of Flag-tagged E2F7 and E2F8 proteins on E2F-binding sites in the promoter of G1/S (E2f1 and Ccne2) and G2/M (Ccna2 and Chek1) genes. The tubulin (Tub) gene, a non-E2F target, demonstrates specific recruitment of Flag-tagged E2F7 and E2F8 to target promoters containing consensus E2F-binding sequences. n = 3 independent transfection–ChIP experiments performed. (c) Immunoblot of transfected Rcho-1 trophoblast stem (TS) cells showing exogenous expression of Flag-tagged E2F7 and E2F8 proteins with tubulin as the control. The arrows indicate tagged protein. (d) Representative ChIP assays in transfected Rcho-1 trophoblast stem cells of G1/S and

    Article Snippet: After antigen retrieval using Target Retrieval Solution (DAKO S1699), 5 μm deparaffinized sections of placenta or liver tissues were incubated with primary antibodies at 4 ◦C overnight against E2F7 1:100 (Abcam ab56022), E2F8 1:50 (polyclonal against residues 576–595 of murine E2F8), BrdU 1:100 (DAKOMO-0744), Ki67 1:100 (BD Pharmingen 550609), phospho-histone 3 (Ser10) 1:100 (Millipore 06-570), cyclin A2 1:100 (Santa Cruz sc-596), cyclin B1 1:100 (Santa Cruz sc-752), E-cadherin 1:100 (Abcam ab53033), p57Kip2 1:100 (Santa Cruz sc-8298; refs 56,57) and placental lactogen 1:200 (PL-1, F. Talamantes).

    Techniques: Control, Western Blot, Transfection, Expressing, Binding Assay

    Figure 7 Cyclin A ablation reinstates genome ploidy of E2f7/E2f8-deficient TGCs and hepatocytes. (a) Images of H&E-stained E10.5 TGCs. The combined loss of Ccna1 and Ccna2 (Plf-A12dko) led to TGCs similar in appearance to control TGCs. The further loss of Ccna1 and Ccna2 in E2f7/E2f8-deficient TGCs (Plf-A1278qko) led to TGCs with a normal (wild-type-like) appearance, in contrast to Plf-78dko TGCs that seemed small or were binucleated (yellow arrows). The lower left insets show higher magnifications of the areas outlined in the main panels. The lower right insets show higher magnifications of the areas outlined in the main panels. (b) Feulgen quantification of genome ploidy in E10.5 TGCs. The intensities (that is, estimated genome content) of 120–140 TGCs were quantified per placenta sample, with n placenta samples analysed per genetic group as indicated. A number of TGCs quadruply deficient for Ccna1, Ccna2, E2f7 and E2f8 reach ploidy levels of 128C and 256C. Control, E2f7f / f;E2f8f / f; Plf-A12, Plfcre/+;Ccna1−/−;Ccna2f / f; Plf-78dko, Plfcre/+;E2f7f / f;E2f8f / f;Plf-A1278qko, Plfcre/+;Ccna1−/−;Ccna2f / f; E2f7f / f;E2f8f / f. (c) Images of H&E-stained 3-month-old liver sections,

    Journal: Nature cell biology

    Article Title: Canonical and atypical E2Fs regulate the mammalian endocycle.

    doi: 10.1038/ncb2595

    Figure Lengend Snippet: Figure 7 Cyclin A ablation reinstates genome ploidy of E2f7/E2f8-deficient TGCs and hepatocytes. (a) Images of H&E-stained E10.5 TGCs. The combined loss of Ccna1 and Ccna2 (Plf-A12dko) led to TGCs similar in appearance to control TGCs. The further loss of Ccna1 and Ccna2 in E2f7/E2f8-deficient TGCs (Plf-A1278qko) led to TGCs with a normal (wild-type-like) appearance, in contrast to Plf-78dko TGCs that seemed small or were binucleated (yellow arrows). The lower left insets show higher magnifications of the areas outlined in the main panels. The lower right insets show higher magnifications of the areas outlined in the main panels. (b) Feulgen quantification of genome ploidy in E10.5 TGCs. The intensities (that is, estimated genome content) of 120–140 TGCs were quantified per placenta sample, with n placenta samples analysed per genetic group as indicated. A number of TGCs quadruply deficient for Ccna1, Ccna2, E2f7 and E2f8 reach ploidy levels of 128C and 256C. Control, E2f7f / f;E2f8f / f; Plf-A12, Plfcre/+;Ccna1−/−;Ccna2f / f; Plf-78dko, Plfcre/+;E2f7f / f;E2f8f / f;Plf-A1278qko, Plfcre/+;Ccna1−/−;Ccna2f / f; E2f7f / f;E2f8f / f. (c) Images of H&E-stained 3-month-old liver sections,

    Article Snippet: After antigen retrieval using Target Retrieval Solution (DAKO S1699), 5 μm deparaffinized sections of placenta or liver tissues were incubated with primary antibodies at 4 ◦C overnight against E2F7 1:100 (Abcam ab56022), E2F8 1:50 (polyclonal against residues 576–595 of murine E2F8), BrdU 1:100 (DAKOMO-0744), Ki67 1:100 (BD Pharmingen 550609), phospho-histone 3 (Ser10) 1:100 (Millipore 06-570), cyclin A2 1:100 (Santa Cruz sc-596), cyclin B1 1:100 (Santa Cruz sc-752), E-cadherin 1:100 (Abcam ab53033), p57Kip2 1:100 (Santa Cruz sc-8298; refs 56,57) and placental lactogen 1:200 (PL-1, F. Talamantes).

    Techniques: Staining, Control